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Recombinant rhesus macaque CD4 Protein, Fc Tag, 100 µg  

Recombinant rhesus macaque CD4 Protein, Fc Tag, 100 µg

Recombinant Rhesus macaque CD4 Protein (Lys 26 - Trp 390), produced in human 293 cells (HEK293), Fc tag (MALS verified)

Synonym
Recombinant rhesus macaque protein, CD4,CD4mut,LEU3

More details

CD4-C5256-100

Availability: within 7 days

325,00 €

Background
T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

Source
Recombinant Rhesus macaque CD4, Fc Tag (CD4-C5256) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # G7N5T8).
Predicted N-terminus: Lys 26

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 67.0 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

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