Recombinant Human CD4 Protein, His tag (MALS verified) DMF Filed, 200 µg

Background
T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

Source
Recombinant Human CD4 /LEU3 Protein, His Tag (LE3-H5228) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782).
Predicted N-terminus: Lys 26

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 41.5 kDa. The protein migrates as 47-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Integrated analysis and validation of ferroptosis-related genes and immune infiltration in acute myocardial infarction"
Wu, Li, Chai et al
BMC Cardiovasc Disord (2024) 24 (1), 123
(2) "Ruxolitinib pharmacokinetics and pharmacodynamics in children with acute and chronic graft versus host disease"
Cook, Dong, Chiang et al
Transplant Cell Ther (2024)
(3) "Analysis of T-lymphocyte subsets and risk factors in children with tuberculosis"
Ma, Wang, Zhao et al
Tuberculosis (Edinb) (2024) 146, 102496
Showing 1-3 of 199364 papers.