Recombinant Human Mucin-1 / MUC-1 Protein, Fc Tag, 100 µg

Background
Membrane mucins have several functions in epithelial cells including cytoprotection, extravasation during metastases, maintenance of luminal structure, and signal transduction. MUC17, contains an extended, repetitive extracellular glycosylation domain and a carboxyl terminus with two EGF-like domains, a SEA module domain, a transmembrane domain, and a cytoplasmic domain with potential serine and tyrosine phosphorylation sites. Interacts via its C-terminus with PDZK1 and this interaction appears important for proper localization. Probably plays a role in maintaining homeostasis on mucosal surfaces.

Source
Recombinant Human Mucin-1, Fc Tag (MU1-H5252) is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Gly 167 (Accession # AAI20976).
Predicted N-terminus: Ser 33

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.8 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "Clinicopathological Factors Affecting Mucin 1, Mucin 2, and Mucin 5AC Staining in Patients who Underwent Resection for Colorectal Cancer"
Anuk, Beseren, Polat et al
J Coll Physicians Surg Pak (2023) 33 (3), 335-340
(2)  "Expression levels of ROS and Atg proteins in the vitreous in rhegmatogenous retinal detachment"
Huang, Gao, Li et al
Int J Ophthalmol (2023) 16 (3), 348-353
(3)  "Smartphone-assisted lab-in-a-tube device using gold nanocluster-based aptasensor for detection of MUC1-overexpressed tumor cells"
Sanati, Esmaeili, Khavani et al
Anal Chim Acta (2023) 1252, 341017
Showing 1-3 of 7231 papers.