Recombinant Cynomolgus CD3 delta Protein, His Tag, 100 µg

Background
T-cell surface glycoprotein CD3 delta, also known as CD3D. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z.

Source
Recombinant Cynomolgus CD3 delta, His Tag (CDD-C52H8) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Ala 105 (Accession # Q95LI8).
Predicted N-terminus: Phe 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.3 kDa. The protein migrates as 25-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "A Case of CD4+ T-Cell Lymphoma With Gamma-Delta Phenotype, Incidentally Manifesting in a Wound Debridement Sample"
Adams, Ehyayee, Ahmed
Am J Dermatopathol (2024)
(2)  "Gamma-delta T-cell acute lymphoblastic lymphoma/leukemia: a report of a rare entity"
George, Kajstura, Evans et al
J Hematop (2024)
(3)  "Aberrant phenotypes of circulating γδ-T cells may be involved in the onset of systemic lupus erythematosus"
Yuan, Wang, Zeng et al
Lupus (2024)
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