Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified), 100µg  

Recombinant Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified), 100µg

Recombinant Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG),  produced in human 293 cells (HEK293), Fc Tag&Fc Tag (MALS verified)

Synonym
Recombinant Human Protein, IL-2 R beta & IL-2 R gamma, IL-2RB & IL-2RG

More details

ILG-H5254-100

Availability: within 7 days

420,00 €

Background
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Source
Recombinant Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) (ILG-H5254) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # NP_000869.1(IL-2RB) & P31785-1(IL-2RG)). Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

Molecular Characterization
Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 51.1 kDa (IL-2RB) and 53.6 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) protein migrates as 65-80 kDa and 90-100 kDa respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL2RG Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) Cecilia K Lindholm, Biochem Biophys Res Commun. 2002. 296(4):929-36.
(2) Xinquan Wang, et al. Science. 2005. 310(5751):1159-63.(3) Deborah J Stauber, et al. Proc Natl Acad Sci U S A. 2006. 103(8):2788-93.
(3) Noguchi M., et al., 1993, Science 262:1877-1880.