Recombinant Human IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified), 100µg

Background
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Source
Recombinant Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (ILG-H5254) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1(IL-2RB) & P31785-1(IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

Molecular Characterization
Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 51.1 kDa (IL-2RB) and 53.6 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) protein migrates as 60-65 kDa and 70-75 kDa respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Immunomodulatory activity of a water-soluble polysaccharide extracted from mussel on cyclophosphamide-induced immunosuppressive mice models"
Xiang, Wang, Chen et al
NPJ Sci Food (2022) 6 (1), 26
(2) "nH115a, a novel inhibitor of the La protein: Effect on expression of multiple RNAs in hepatitis B virus-infected hepatoma cells and embryotoxicity profile"
Pan, Tang
Acta Virol (2022) 66 (1), 65-76
(3) "Immune-Enhancing Effect of Submerged Culture of Ceriporia lacerata Mycelia on Cyclophosphamide-Induced Immunosuppressed Mice and the Underlying Mechanisms in Macrophages"
Hwang, Lee, Pham et al
Int J Mol Sci (2022) 23 (2)
Showing 1-3 of 275 papers.