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Recombinant Human Fc gamma RIIB / CD32b Protein, Strep Tag (MALS & SPR verified), 50 µg  

Recombinant Human Fc gamma RIIB / CD32b Protein, Strep Tag (MALS & SPR verified), 50 µg

Recombinant Human FcGR2B / CD32b Protein, Ala 46 - Pro 217, produced in human 293 cells, Strep Tag (SPR & BLI & MALS verified)

Synonym
Recombinant, human, protein CD32b,FCGR2B,CD32,FCG2 ,FCGR2B,IGFR2

More details

CDB-H5284-50

Availability: within 7 days

364,00 €

Background
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized.
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies..

Source
Recombinant 

Human CD32b Protein, Strep Tag(CDB-H5284) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1 ). Predicted N-terminus: Ala 46


Molecular Characterization
This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 22.7 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. NormallyTrehalose are added as protectants before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

  • Wang, Sun, Zuo et al. (2021)"Homogeneously high expression of CD32b makes it a potential target for CAR-T therapy for chronic lymphocytic leukemia" J Hematol Oncol 14 (1), 149.
  • Trend, Leffler, Teige et al. (2020) "FcγRIIb Expression Is Decreased on Naive and Marginal Zone-Like B Cells From Females With Multiple Sclerosis." Front Immunol 11, 614492
  • Tang, Zhang, Hung et al. (2021) "DPP4/CD32b/NF-κB Circuit: A Novel Druggable Target for Inhibiting CRP-Driven Diabetic Nephropathy." Mol Ther 29 (1), 365-375

Showing 1-3 of 45 papers.

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