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Recombinant human CDK2/CycA2, active protein kinase,10 µg  

Recombinant human CDK2/CycA2, active protein kinase,10 µg

Recombinant, human Cyclin-dependent kinase 2 in complex with cyclin A2, (CDK2/CycA2) active enzyme

Alternate names: recombinant human protein kinase CDK2, p33, Cyclin dependent kinase 2, p33 protein kinase, cdc2 related protein kianse, cell division protein kinase 2, p33/CyclinA2, CCNA2, CCN1

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Availability: on stock

465,00 €

Cdks are the catalytic subunits of serine/threonine protein kinases which are involved in the progression through the cell cycle. The catalytic activity of these kinases requires interaction with cyclins, which are synthesized and degraded during the cell cycle in a cyclical fashion. The synthesis of Cyclin A increases towards the end of the G1 phase. CDK2 associated with cyclin A partly controls DNA replication, phosphorylates proteins required for completion and exit from S phase like the Minichromosome maintenance protein 4 (MCM4), modifies upstream regulators of cyclin A (retinoblastoma protein, E2F) and regulates cell-cycle checkpoint proteins (p53, p21 and MDM2).

Recombinant human cyclin-dependent protein kinase CDK2, coexpressed in complex with Cyclin A2.

Theoretical MW (CDK2/CycA2): 60.2/74.9 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Hepes, pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.27 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 67,000 pmol/mg min

Ordering information: shipped on dry ice

Entrez Gene ID: 1017/890 
UniProtKB:  P24941/P20248

Product specific literature references:

Gu Y, Rosenblatt J, Morgan DO (1992) "Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15." EMBO J. 11(11):3995-4005

Cardoso MC, Leonhardt H, Nadal-Ginard B (1993) "Reversal of terminal differentiation and control of DNA replication: cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication." Cell 74(6):979-92

Gyuris J, Golemis E, Chertkov H, Brent R (1993) "Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2." Cell 75(4):791-803

Gu Y, Turck CW, Morgan DO (1993) "Inhibition of CDK2 activity in vivo by an associated 20K regulatory subunit." Nature 366(6456):707-10

Connell-Crowley L, Solomon MJ, Wei N, Harper JW (1993) "Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro" Mol Biol Cell. 4(1):79-92

Pagano M, Pepperkok R, Lukas J, Baldin V, Ansorge W, Bartek J, Draetta G (1993) "Regulation of the cell cycle by the cdk2 protein kinase in cultured human fibroblasts." J Cell Biol. 121(1):101-11

Jeffrey PD, Russo AA, Polyak K, Gibbs E, Hurwitz J, Massague J, Pavletich NP (1995) "Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex." Nature 376(6538):313-20

Radzio-Andzelm E, Lew J, Taylor S (1995) "Bound to activate: conformational consequences of cyclin binding to CDK2" Structure 3(11):1135-41

Koyama H, Raines EW, Bornfeldt KE, Roberts JM, Ross R (1996) "Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 inhibitors." Cell 87(6):1069-78

Guadagno TM, Newport JW (1996) "Cdk2 kinase is required for entry into mitosis as a positive regulator of Cdc2-cyclin B kinase activity" Cell. 84(1):73-82

Russo AA, Jeffrey PD, Patten AK, Massague J, Pavletich NP (1996) "Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor bound to the cyclin A-Cdk2 complex" Nature 382(6589):325-31

Hua XH, Yan H, Newport J (1997) "A role for Cdk2 kinase in negatively regulating DNA replication during S phase of the cell cycle." J Cell Biol. 137(1):183-92

Huang H, Regan KM, Lou Z, Chen J, Tindall DJ (2006) "CDK2-dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage." Science 314(5797):294-7

Canela N, Orzaez M, Fucho R, Mateo F, Gutierrez R, Pineda-Lucena A, Bachs O, Perez-Paya E (2006) "Identification of an hexapeptide that binds to a surface pocket in cyclin A and inhibits the catalytic activity of the complex cyclin-dependent kinase 2-cyclin A." J Biol Chem. 2006 Sep 25