kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human TNF-alpha Protein, His Tag, active trimer (MALS verified), 100 µg  

Recombinant Human TNF-alpha Protein, His Tag, active trimer (MALS verified), 100 µg

Recombinant Human TNF-alpha Protein, AA Val 77 - Leu 233, expressed in human 293 cells (HEK293), His Tag, active trimer (MALS verified)

Synonym: Recombinant human protein, DIF,TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

More details


Availability: within 7 days

403,00 €

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Recombinant Human TNF-alpha, His Tag (active trimer, MALS verified) (TNA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).
Predicted N-terminus: Val 77

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

See Certificate of Analysis for reconstitution instructions and specific concentrations.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

(1) "Does TNF-α 308 G/A (rs1800629) gene polymorphism associate with liver and pancreas disorders in Iraqi adults with beta thalassemia major?"
Luaibi, Mohammed
Hum Antibodies (2023)
(2) "Mechanism of lysine oxidase-like 1 promoting synovial inflammation mediating rheumatoid arthritis development"
Hu, Liu, Xu et al
Aging (Albany NY) (2024) 15
(3) "ZIP4 upregulation aggravates nucleus pulposus cell degradation by promoting inflammation and oxidative stress by mediating the HDAC4-FoxO3a axis"
Shen, Li, Wang et al
Aging (Albany NY) (2024) 15
Showing 1-3 of 263072 papers.

The following products could also be interesting for you: