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Recombinant Mouse LIF Protein, His tag, 50µg  

Recombinant Mouse LIF Protein, His tag, 50µg

Recombinant mouse Leukemia inhibitory factor / LIF Protein (Ser 24 - Phe 203), produced in human 293 cells (HEK293), His tag

Synonyms: Recombinant human protein, LIF,CDF,DIA,HILDA,MLPLI, interleukin 6 class

More details

LIF-M5227-50

Availability: within 7 days

360,00 €

Background
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Source
Recombinant mouse LIF Protein (Mouse LIF, His Tag) Ser 24 - Phe 203 (Accession # P09056) was produced in human 293 cells (HEK293)

Molecular Characterization
Mouse LIF, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 20.7 kDa. The predicted N-terminus is Ser 24. DTT-reduced Protein migrates as 33-45 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse LIF, His Tag by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Hu W,et al., 2007, Nature, 450 (7170): 721-4.
(2) Kawahara Y,et al., 2009, PLoS One, 4 (7): e6343.
(3) Patterson PH (1994). Proc. Natl. Acad. Sci. U.S.A. 91 (17).