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Recombinant Mouse LIF Protein, His Tag (MALS verified), 50µg  

Recombinant Mouse LIF Protein, His Tag (MALS verified), 50µg

Recombinant mouse Leukemia inhibitory factor / LIF Protein (Ser 24 - Phe 203), produced in human 293 cells (HEK293), His Tag (MALS verified)

Synonyms: Recombinant human protein, LIF, CDF, DIA, HILDA, MLPLI, interleukin 6 class

More details

LIF-M5227-50

Availability: within 7 days

360,00 €

Background
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Source
Recombinant Mouse LIF, His Tag (LIF-M5227) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1).
Predicted N-terminus: Ser 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.8 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse LIF, His Tag by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Jumonji domain-containing protein-3 (JMJD3/Kdm6b) is critical for normal ovarian function and female fertility"
Roy, Sinha, Huang et al
Endocrinology (2022)
(2) "Differentiation of mouse embryonic stem cells into cells with spermatogonia-like morphology with chemical intervention-dependent increased gene expression of LIM homeobox 1 (Lhx1)"
Moshfegh, Rambow, Domenig et al
Stem Cell Res (2022) 61, 102780
(3) "Formation of LiF-rich Cathode-Electrolyte Interphase by Electrolyte Reduction"
Wang, Bai, Ji et al
Angew Chem Int Ed Engl (2022)
Showing 1-3 of 10515 papers.