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Recombinant Human IL-12B / NKSF2 / p40 Protein, Fc Tag, 50µg  

Recombinant Human IL-12B / NKSF2 / p40 Protein, Fc Tag, 50µg

Recombinant Human IL12B /P40 Protein (Ile 23 - Ser 328), expressed in human 293 cells (HEK293), Fc Tag

Synonyms: Recombinant human protein, IL12B, CLMF, CLMF2, NKSF, NKSF2, p40

More details

NK2-H5258-50

Availability: within 7 days

432,00 €

Background
Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.

Source
Recombinant Human IL12B /P40 Protein, Fc Tag (NK2-H5258) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499).
Predicted N-terminus: Ile 23

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 61.3 kDa. The protein migrates as 66-76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh IL12B /P40 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Association of interleukin-12B gene polymorphisms and mRNA expression with preeclampsia"
Duan, Wang, Li et al
Eur J Obstet Gynecol Reprod Biol (2021) 269, 77-82
(2) "A Meta-Analysis for Association of XRCC3 rs861539, MTHFR rs1801133, IL-6 rs1800795, IL-12B rs3212227, TNF-α rs1800629, and TLR9 rs352140 Polymorphisms with Susceptibility to Cervical Carcinoma"
Parsaeian, Asadian, Karimi-Zarchi et al
Asian Pac J Cancer Prev (2021) 22 (11), 3419-3431
(3) "Primary alterations during the development of hidradenitis suppurativa"
Dajnoki, Somogyi, Medgyesi et al
J Eur Acad Dermatol Venereol (2021)
Showing 1-3 of 291 papers.