Recombinant Human IL-1 beta / IL-1F2 Protein, His Tag (MALS verified), 20 µg

Background
Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Source
Recombinant Human IL-1 beta, His Tag (ILB-H51H3) is expressed from E.coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1).
Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 19.4 kDa. The protein migrates as 19-20 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "IL-1β restrains its own apoptosis-promoting activity via NF-κB (RelA) Ser276/p62-mediated elimination of damaged mitochondria in Chondrocytes"
Hu, Zhang, Zhao
Biosci Biotechnol Biochem (2025)
(2) "Wildfire Smoke Induces Eye Surface Inflammation and Tear Film Changes in a Human Experimental Model"
Jaiswal, Golebiowski, Porter et al
Transl Vis Sci Technol (2025) 14 (9), 13
(3) "Comprehensive genetic analysis of poorly differentiated gastric cancer in young females"
Nakazawa, Yokobori, Morishita et al
Ann Gastroenterol Surg (2025) 9 (5), 926-932
Showing 1-3 of 63174 papers.