Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human IL-1 beta / IL-1F2 Protein, His Tag (MALS verified), 50 µg  

Recombinant Human IL-1 beta / IL-1F2 Protein, His Tag (MALS verified), 50 µg

Recombinant Human IL-1 beta / IL-1F2 Protein, AA Ala 117 - Ser 269, expressed from E.coli cells, His Tag (MALS verified)

Synonym
Recombinant, IL1B, IL-1BETA, IL1F2, IL-1β

 

More details

ILB-H51H3-50

Availability: within 7 days

403,00 €

Background
Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Source
Recombinant Human IL-1 beta, His Tag (ILB-H51H3) is expressed from E.coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1).
Predicted N-terminus: Met

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 19.3 kDa. The protein migrates as 19-20 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The identification of new roles for nicotinamide mononucleotide after spinal cord injury in mice: an RNA-seq and global gene expression study"
Zhang, Li, Bai et al
Front Cell Neurosci (2023) 17, 1323566
(2) "Comparison Shows that Multiplex Polymerase Chain Reaction Identifies Infection-associated Urinary Biomarker-positive Urinary Tract Infections That Are Missed by Standard Urine Culture"
Haley, Luke, Mathur et al
Eur Urol Open Sci (2023) 58, 73-81
(3) "Prenatal alcohol exposure promotes NLRP3 inflammasome-dependent immune actions following morphine treatment and paradoxically prolongs nerve injury-induced pathological pain in female mice"
Noor, Sun, Pasmay et al
Alcohol Clin Exp Res (Hoboken) (2023) 47 (12), 2262-2277
Showing 1-3 of 58345 papers.