Recombinant Human IL-31 RA Protein, His Tag (MALS & SPR verified), 100 µg

Background
Interleukin-31 receptor subunit alpha is a protein that in humans is encoded by the IL31RA gene, also known as IL-31 receptor subunit alpha, IL-31RA, GLM-R, Gp130-like receptor, CRL3, GPL. Oncostatin M receptor (OSMR) and IL31RA form the heterodimeric receptor through which IL31 signals. IL31RA is a strong activator of STAT3 and STAT5, whereas STAT1 is only marginally tyrosine-phosphorylated. dditionally, demonstrate Jak1 binding to GPL and its activation in heteromeric complexes with the OSMRbeta but also in a homomeric receptor complex.

Source
Recombinant Human IL-31 RA, His Tag (ILA-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 519 (Accession # Q8NI17-1).
Predicted N-terminus: Ala 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.0 kDa. The protein migrates as 90-110 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Chronic Prurigo"
Thünemann, Müller, Steinbrink et al
J Dtsch Dermatol Ges (2024)
(2) "TRPV1 Channel in Human Eosinophils: Functional Expression and Inflammatory Modulation"
Weihrauch, Gray, Wiebe et al
Int J Mol Sci (2024) 25 (3)
(3) "Infiltration analysis of eosinophils and basophils and co-expression of CD69, CD63, IL-31 and IgE in patients with bullous and non-bullous pemphigoid"
Kotnik, Langner, Meyer et al
J Eur Acad Dermatol Venereol (2024) 38 (3), e278-e281
Showing 1-3 of 12 papers.