Recombinant Human IL-27 Ra / TCCR Protein, Fc Tag, 100 µg

Background
Interleukin-27 receptor subunit alpha ( IL27RA) is also known as Cytokine receptor WSX-1, Cytokine receptor-like 1, Type I T-cell cytokine receptor (TCCR), ZcytoR1, IL27RA belongs to the type I cytokine receptor family and type 2 subfamily. IL27RA contains three fibronectin type-III domains. IL27RA is highly expressed in lymphoid tissues such as spleen, lymph nodes and peripheral blood leukocytes. IL27RA is receptor for IL27, but requires IL6ST/gp130 to mediate signal transduction in response to IL27. IL27RA involved in the regulation of Th1-type immune responses. IL27RA also appears to be involved in innate defense mechanisms.

Source
Recombinant Human IL-27 Ra, Fc Tag (ILA-H5254) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Arg 514 (Accession # AAH28003).
Predicted N-terminus: Gln 33

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 78.7 kDa. The protein migrates as 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Selective IL-27 production by intestinal regulatory T cells permits gut-specific regulation of Th17 immunity"
Lin, Wu, Cho et al
bioRxiv (2023)
(2) "Epstein-Barr virus-induced gene 3 commits human mesenchymal stem cells to differentiate into chondrocytes via endoplasmic reticulum stress sensor"
Zhang, Yamagata, Iwata et al
PLoS One (2022) 17 (12), e0279584
(3) "Elevated expression of interleukin-27, IL-35, and decreased IL-12 in patients with thyroid-associated ophthalmopathy"
Zhang, Zhang, Xu et al
Graefes Arch Clin Exp Ophthalmol (2023) 261 (4), 1091-1100
Showing 1-3 of 47 papers.