Recombinant Mouse IL-2 R beta Protein, His Tag, 50 µg

Background
Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit.
CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

Source
Recombinant Mouse IL-2 R beta, His Tag (ILA-M52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 240 (Accession # P16297-1).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 27.1 kDa. The protein migrates as 42-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Evaluation of the mechanism of Gong Ying San activity on dairy cows mastitis by network pharmacology and metabolomics analysis"
Gao, Tang, Ma et al
PLoS One (2024) 19 (4), e0299234
(2) "Cytokine Plasma Levels in Breast Cancer Patients, Before and After Surgery"
Kontomanolis, Tsigalou, Mitrakas et al
J Interferon Cytokine Res (2024) 44 (3), 135-142
(3) "Low pre-existing endemic human coronavirus (HCoV-NL63)-specific T cell frequencies are associated with impaired SARS-CoV-2-specific T cell responses in people living with HIV"
Ng'uni, Musale, Nkosi et al
Front Immunol (2023) 14, 1291048
Showing 1-3 of 673 papers.