Recombinant Mouse IL-2 R beta Protein, His Tag, 50 µg

Background
Interleukin-2 receptor subunit beta (IL2RB) is also known as CD122, which Belongs to the type I cytokine receptor family. Type 4 subfamily. IL2RB contains 1 fibronectin type-III domain. IL2RB / CD122 is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2.

Source
Recombinant Mouse IL-2 R beta, His Tag (ILA-M52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 240 (Accession # P16297-1).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.1 kDa. The protein migrates as 42-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Upregulated Expression of IL2RB Causes Disorder of Immune Microenvironment in Patients with Kawasaki Disease"
Liao, Ke, Long et al
Biomed Res Int (2022) 2022, 2114699
(2) "Effects of dietary Glycyrrhiza polysaccharide supplementation on growth performance, intestinal antioxidants, immunity and microbiota in weaned piglets"
Zhang, Li, Zhao et al
Anim Biotechnol (2022)
(3) "The early faecal microbiota transfer alters bile acid circulation and amino acid transport of the small intestine in piglets"
Teng, Sun, Song et al
J Anim Physiol Anim Nutr (Berl) (2022)
Showing 1-3 of 610 papers.