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Recombinant Canine IL-2 R beta Protein, His Tag, 50 µg  

Recombinant Canine IL-2 R beta Protein, His Tag, 50 µg

Recombinant Canine IL-2 R beta Protein, AA Asn 30 - Pro 246, expressed from human 293 cells (HEK293), His Tag

Synonym
Recombinant Canine Protein, IL2RB, RP5-1170K4.6, CD122, P70-75

More details

ILA-C52H5-50

Availability: within 7 days

455,00 €

Background
Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit.
CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

Source
Recombinant Canine IL-2 R beta, His Tag(ILA-C52H5) is expressed from human 293 cells (HEK293). It contains AA Asn 30 - Pro 246 (Accession # NP_001273780.1 ).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.0 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Immune-enhancing effect of hydrolyzed and fermented Platycodon grandiflorum extract in cyclophosphamide-induced immunosuppressed BALB/c mice"
Lee, Kim, Jung et al
Nutr Res Pract (2023) 17 (2), 206-217
(2) "Serum cytokines-based biomarkers in the diagnosis and monitoring of therapeutic response in patients with major depressive disorder"
Xu, Liang, Sun et al
Int Immunopharmacol (2023) 118, 110108
(3) "Praziquantel promotes protection against Schistosoma japonicum infection in mice"
Shao, Gui, Lu et al
Acta Trop (2023) 241, 106874
Showing 1-3 of 650 papers.