Recombinant Biotinylated Cynomolgus IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified), 25 µg

Background
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Source
Recombinant Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag (ILG-C82F4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Thr 240 (IL-2RB) & Leu 23 - Pro 255 (IL-2RG) (Accession # Q38J85-1(IL-2RB) & Q38JL2-1(IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Leu 23 (IL-2RG)

Molecular Characterization
Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB andIL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 55.4 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 70-85 kDa and 90-110 kDa respectively due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Immune-Enhancing Effect of Submerged Culture of Ceriporia lacerata Mycelia on Cyclophosphamide-Induced Immunosuppressed Mice and the Underlying Mechanisms in Macrophages"
Hwang, Lee, Pham et al
Int J Mol Sci (2022) 23 (2)
(2) "TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer"
Beatson, Parente-Pereira, Halim et al
Cell Rep Med (2021) 2 (12), 100473
(3) "Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice"
Cai, Wusiman, Gu et al
Food Funct (2021) 12 (20), 9693-9707
Showing 1-3 of 272 papers.