Recombinant, human ZAP70 protein kinase, 10 µg

Background: ZAP70 (Zeta-chain associated protein kinase) is a 70 kDa member of the Syk family kinase predominantly involved in T cell receptor (TCR) signaling. However, recent findings have revealed involvement of ZAP70 in B cell receptor (BCR) signaling especially in leukemic and primary B cells too. The cytosolic protein kinase is characterized by the presence of tandem Src-homology 2 (SH2) domains preceding a highly conserved catalytic domain. Following TCR stimulation ZAP70 is recruited to the plasma membrane mediated by binding of the two SH2 domains of ZAP70 to the doubly phosphorylated immunoreceptor tyrosine-based activation motif (ITAMs) of the TCR.  This translocation process enables ZAP70 to be activated by Lck mediated phosphorylation on numerous tyrosine residues, but also contributes to relieve an autoinhibited conformation of the kinase. Phosphorylated ZAP70 in turn activates intermediates such as linker for activation (LAT) and SLP-76 leading to calcium mobilization and activation of downstream signaling pathways (MAPK and PI3 kinase).  ZAP70 controls cytoskeleton modifications, adhesion and mobility of T lymphocytes and is involved in human thymocyte development. Defects in ZAP70 signaling have been associated with autoimmunity  and cancer.  

human Zeta-chain-associated protein kinase 70 , protein tyrosine kinase, full lenght, human recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site<p >Theoretical MW : 99.496 kDa (fusion proteins)

Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.26 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 76,000 pmol/mg min

Entrez Gene ID: 7535
UniProtKB: P43403

Ordering information: shipped on dry ice