Recombinant, human TANK-binding kinase 1 (TBK1), untagged, 10µg

Background: TBK1 (tumor necrosis factor (TNF) receptor associated factor NF-κB activator (TANK)-binding kinase 1) is an IKK-related serine/threonine kinase, expressed in both hematopoietic and non-hematopoietic compartments. TBK1 contains four domains: a serine/threonine kinase domain at its N-terminal, a ubiquitin-like domain and two coiled-coil domains. Activation is thought to occur via trans-autoactivation, in response to adaptor proteins that shuttle TBK1 to specific signaling complexes and direct subcellular localizations, such as to the ER-Golgi compartments. TBK1 is involved in the activation of various cellular pathways leading to IFN and pro-inflammatory cytokine production following infection, autophagic degradation of protein aggregates or pathogens, and homeostatic cellular functions such as cell growth and proliferation.

Human TBK1 (TANK-binding kinase 1), amino acids M1-L729, recombinant and active enzyme, untagged, expressed in Sf9 insect cells

Theoretical MW: 84.225 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.132 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 146.000 pmol/mg min

Entrez Gene ID 29110 
UniProtKB Q9UHD2

Ordering information: shipped on dry ice

Product specific literature

Ahmad L, Zhang SY, Casanova JL, Sancho-Shimizu V. (2016) “Human TBK1: A Gatekeeper of Neuroinflammation.” Trends Mol Med. 22(6):511-527.
Oakes JA, Davies MC, Collins MO. (2017) “TBK1: a new player in ALS linking autophagy and neuroinflammation.” Mol Brain. 2;10(1):5.
Louis C, Burns C, Wicks I (2018) “TANK-Binding Kinase 1-Dependent Responses in Health and Autoimmunity.” Front Immunol. 2018 Mar 6;9:434.