AMPK peptide substrate, 5 mg

The synthetic peptide HMRSAMSGLHLVKRR can be used as a substrate for 5\'-AMP-activated protein kinase (AMPK) in in vitro kinase assays. It is phosphorylated by AMPK with a Km of 33.3 µM and a Vmax of 8.1 µmol/min/mg.

AMPK substrate peptide :    order-no: PEP-AMPK-005
Amino acid sequence.:        HMRSAMSGLHLVKRR-amid (TFA salt)
Molecular Weight:                1778.2 g/mol
Shipment conditions:          ambient temperature
Long Term Storage:            -20°C (AVOID MULTIPLE FREEZE/THAW CYCLES)
Purity:                                     >90% (HPLC, MS)
Appearance:                         Lyophilized white solid
Handling:                              Reconstitution in H2O dest.

Product specific literature references:

Michell BJ, Stapleton D, Mitchelhill KI, House CM, Katsis F, Witters LA, Kemp BE. (1996) "Isoform-specific purification and substrate specificity of the 5\'-AMP-activated protein kinase." J. Biol. Chem. 271(45):28445-50

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.