AMPK peptide substrate (AMARA), 1 mg

The synthetic peptide AMARAASLARRR can be used as a substrate for 5´-AMP-activated protein kinase (AMPK) in in vitro kinase assays. It is phosphorylated by human AMPK in the presence of 200 microM 5´-AMP with a Km of 10 µM and a Vmax of 1.67 nM/min/mg.

AMPK substrate peptide :  PEP-AMARA-001
Amino acid sequence.:      AMARAASLARRR
Molecular Weight:             1330.0 g/mol
Shipment conditions:          ambient temperature
Long Term Storage:          -20°C (AVOID MULTIPLE FREEZE/THAW CYCLES)
Purity:                                >90% (HPLC)
Appearance:                      Lyophilized white solid
Handling:                           Reconstitution in water dest.

Product specific literature references:

Sakamoto K1, Göransson O, Hardie DG, Alessi DR. (2004) “Activity of LKB1 and AMPK-related kinases in skeletal muscle: effects of contraction, phenformin, and AICAR.” Am J Physiol Endocrinol Metab.;287(2): E310-7

Salt IP, Johnson G, Ashcroft SJ, Hardie DG.(1998) “AMP-activated protein kinase is activated by low glucose in cell lines derived from pancreatic beta cells, and may regulate insulin release.” Biochem J.;335 ( Pt 3):533-9.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.