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The synthetic peptide AMARAASLARRR can be used as a substrate for 5´-AMP-activated protein kinase (AMPK) in in vitro kinase assays. It is phosphorylated by human AMPK in the presence of 200 microM 5´-AMP with a Km of 10 µM and a Vmax of 1.67 nM/min/mg.
AMPK substrate peptide : PEP-AMARA-001
Amino acid sequence.: AMARAASLARRR
Molecular Weight: 1330.0 g/mol
Shipment conditions: ambient temperature
Long Term Storage: -20°C (AVOID MULTIPLE FREEZE/THAW CYCLES)
Purity: >90% (HPLC)
Appearance: Lyophilized white solid
Handling: Reconstitution in water dest.
Sakamoto K1, Göransson O, Hardie DG, Alessi DR. (2004) “Activity of LKB1 and AMPK-related kinases in skeletal muscle: effects of contraction, phenformin, and AICAR.” Am J Physiol Endocrinol Metab.;287(2): E310-7
Salt IP, Johnson G, Ashcroft SJ, Hardie DG.(1998) “AMP-activated protein kinase is activated by low glucose in cell lines derived from pancreatic beta cells, and may regulate insulin release.” Biochem J.;335 ( Pt 3):533-9.
PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.
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