TC-PTP, 5 µg

TC-PTP is an intracellular protein tyrosine phosphatase which is mostly expressed in hematopoietic tissues. The phosphatase exists as two splice variants in human, carrying either a nuclear localization signal (45 kDa form) or an endoplasmic reticulum (ER) retention motif (48 kDa form) at the C-terminus. Both forms consist of a classical PTP catalytic domain at the N-terminus, which is closely related to the catalytic region of PTP1B. TC-PTP functions as a negative regulator of the JAK/STAT cytokine signaling pathway and is involved in the development of hematopoietic lineages. Furthermore, TC -PTP is phosphorylated on serine-304 by CDKs during mitosis. The absence of TC-PTP results in decreased proliferation and slower G1 phase progression during the cell cycle and is characterized by defective NFκB signaling. TC-PTP is also involved in the regulation of several RTKs. For instance dephosphorylation of EGFR by TC-PTP results in decreased downstream activation of PI3K and Akt signaling. Altered TC-PTP expression levels are associated with immunological diseases such as autoimmune disorders and cancer.

Protein information: Purified recombinant human protein tyrosine phosphatase (aa 1-354, NM_002828), catalytic domain, expressed in E.coli. N-terminally tagged with GST. Purified by affinity chromatography. Molecular weight: 68.1 kDA, Protein concentration: 0.2 mg/ml, Purity: > 90% by SDS PAGE

Ordering information: shipped on dry ice

Product specific literature:

Doody KM, Bourdeau A, Tremblay (2009) “T-cell protein tyrosine phosphatase is a key regulator in immune cell signaling: lessons from the knockout mouse model and implications in human disease.” Immunol Rev.228(1):325-41.

Stuible M, Doody KM, Tremblay ML.(2008) “PTP1B and TC-PTP: regulators of transformation and tumorigenesis. Cancer Metastasis Rev. 27(2):215-30.

Bourdeau A, Dubé N, Heinonen KM, Théberge JF, Doody KM, Tremblay ML.(2007) “TC-PTP-deficient bone marrow stromal cells fail to support normal B lymphopoiesis due to abnormal secretion of interferon-{gamma}.” Blood. 2007 May 15;109(10):4220-8.