Recombinant Human FcRn / FCGRT&B2M Heterodimer Protein, Strep Tag&Tag Free (MALS & SPR verified), 50µg

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
Recombinant Human FCGRT&B2M Heterodimer Protein, Strep Tag&Tag Free(FCM-H5283) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)).Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization
Human FCGRT&B2M Heterodimer Protein, Strep Tag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 33.8 kDa (FCGRT) and 11.7 kDa (B2M). Subunit FCGRT is fused with Strep II-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag. The reducing (R) protein migrates as 12 kDa (FCGRT) and 38-45 kDa (B2M) respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) Agrawal, Kumar, Chaudhary et al. (2023)"Allele mining in gene encoding fc fragment of IgG receptor, transporter, alpha (FCGRT) and association of nucleotide variants with passive transfer of immunity in neonatal buffalo calves" Mol Biol Rep (2023) 50 (4), 3429-3438
(2) Chen, Qu, Liu et al. (2022) "Human FcRn Is a Two-in-One Attachment-Uncoating Receptor for Echovirus 18"mBio (2022) 13 (4), e0116622 Mol Biol Rep (2022) 49 (5), 3773-3781
(3) Agrawal, Kumar, Chaudhary et al. (2022)"Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis)" Mol Biol Rep (2022) 49 (5), 3773-3781
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