Recombinant human IL2RB /CD122 Protein, Fc Tag, 50µg

Background
Interleukin-2 receptor subunit beta (IL2RB) is also known as CD122, which Belongs to the type I cytokine receptor family. Type 4 subfamily. IL2RB contains 1 fibronectin type-III domain. IL2RB / CD122 is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2.

Source
Recombinant Human IL2RB /CD122 Protein, With C-Fc Tag (rhIL2RB/CD122 Fc Chimera) Ala 27 - Asp 239 (Accession # NP_000869.1) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.7 kDa. The protein migrates as 58-76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL2RB/CD122 Fc Chimera by the LAL method.

Purity
>92% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human IL-15, Tag Free (Cat. No. IL5-H4217) at 10 μg/mL (100 μL/well) can bind Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) with a linear range of 0.156-2.5 μg/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilized Human IL-2 R beta, Fc Tag (Cat. No. ILB-H5253) at 2 μg/mL (100 μL/well) can bind Human IL-2, Tag Free (Cat. No. IL2-H4216) with a linear range of 0.156-2.5 μg/mL (Routinely tested).

References

(1) Mulloy J.C., et al., 1996, J. Virol. 70:3599-3605.
(2) Lindholm C.K., 2002, Biochem. Biophys. Res. Commun. 296:929-936.