- Remove this product from my favorite's list.
- Add this product to my list of favorites.
Products
Newsletter
Background
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Source
Recombinant Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (MALS verified) (CDD-M5219) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1(CD3E) & P04235-1(CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
Molecular Characterization
Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.1 kDa (CD3E) and 14.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 18-19 kDa and 25-32 kDa under reducing (R) condition, and 38-47 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Bioactivity
Please refer to product data sheet.
(1) "Identification of Genes Associated with Decreasing Abundance of Monocytes in Long-Term Peritoneal Dialysis Patients"
Zhang, Jin, Wang et al
Int J Gen Med (2023) 16, 5017-5030
(2) "Correlation Analysis of Prognostic Gene Expression, Tumor Microenvironment, and Tumor-Infiltrating Immune Cells in Ovarian Cancer"
Li, Yang, Ling et al
Dis Markers (2023) 2023, 9672158
(3) "Leveraging Genomic and Bioinformatic Analysis to Enhance Drug Repositioning for Dermatomyositis"
Irham, Adikusuma, La'ah et al
Bioengineering (Basel) (2023) 10 (8)
Showing 1-3 of 49 papers.
Recombinant human CD23 / Fc epsilon RII Protein, His tag, 100µg - 364,00 €
Recombinant human CD62E / E-Selectin Protein, His tag, 200µg - 390,00 €
Recombinant human CD33 / SIGLEC-3 Protein, His tag, 100µg - 442,00 €
Recombinant Biotinylated Human Siglec-3 / CD33 Protein, Avi Tag (Avitag™), 25µg - 325,00 €
Welcome Login
Contact us
Follow us