Recombinant Human Glypican 1 / GPC1 Protein, His Tag, 100 µg

Background
Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Source
Recombinant Human Glypican 1, His Tag (GP1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1).
Predicted N-terminus: Asp 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 58.0 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>85% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "Steric Hindrance-Mediated Enzymatic Reaction Enable Homogeneous Dual Fluorescence Indicators Aptasensing of Hepatocellular Carcinoma CTCs"
Jiang, Zhan, Peng et al
Anal Chem (2024) 96 (26), 10705-10713
(2)  "Differential extracellular vesicle concentration and their biomarker expression of integrin αv/β5, EpCAM, and glypican-1 in pancreatic cancer models"
Jacobson, Ha, Tani et al
Sci Rep (2024) 14 (1), 14273
(3)  "Internalization of PEI-based complexes in transient transfection of HEK293 cells is triggered by coalescence of membrane heparan sulfate proteoglycans like Glypican-4"
Pérez-Rubio, Vendrell-Flotats, Romero et al
Biomed Pharmacother (2024) 176, 116893
Showing 1-3 of 1692 papers.