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Recombinant Biotinylated Human EpCAM / TROP1, His-Tag, 25 µg  

Recombinant Biotinylated Human EpCAM / TROP1, His-Tag, 25 µg

MABSol® Recombinant Biotinylated Human EpCAM / TROP1 Protein (EPM-H8223), expressed from human HEK293 cells, His-Tag, ultra sensitivity (primary amine labeling)

Synonyms: recombinant, human, biotinylated, protein, EPCAM, TACSTD1, TROP1, CD326, DIAR5, EGP2, EGP314, EGP40, HNPCC8, , biotinylated EpCAM, btn-EpCAM

More details

EPM-H8223-25

348,00 €

Background: EpCAM is also known as CO171A,; EGP; EGP40; GA7332; KSA; M4S; MIC18; MK1; TROP1; hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity.[1] The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy.[2] Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported.[3] And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.[4]

The primary amine of rhEpCAM was labeled by biotin without decreasing its bioactivity, and has been used successfully for easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated probes. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination.

Source
MABSol® Biotinylated Human EpCAM, His Tag, primary amine labeling (EPM-H8223) is expressed from human HEK293 cells. It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1). It is the biotinylated form of Human EpCAM, His Tag (EPM-H5223).
Predicted N-terminus: Gln 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.0 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29 kDa. The protein migrates as 33-36 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1)Linnenbach, et al., 1993, Mol Cell Biol 13 (3): 1507–15.
(2)Akita H, et al., 2011, Oncogene., 30 (31): 3468-76.
(3)Winter MJ, et al., 2007, Mol Cell Biochem. 302(1-2):19-26.
4)Schmidt M, et al., 2011, Breast Cancer Res Treat. 125(3):637-46.