Recombinant Biotinylated Mouse FcRn / FCGRT & B2M, Avi Tag (Avitag™), 25 µg

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
MABSol® Biotinylated Mouse FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (FCM-M82W6) is expressed from human HEK293 cells. It contains AA Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q61559-1(FCGRT) & P01887-1(B2M)).
Predicted N-terminus: Ser 22 (FCGRT) & Ile 21 (B2M)

Molecular Characterization
Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) "Human FcRn Is a Two-in-One Attachment-Uncoating Receptor for Echovirus 18"
Chen, Qu, Liu et al
mBio (2022)
(2) "Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis)"
Agrawal, Kumar, Chaudhary et al
Mol Biol Rep (2022) 49 (5), 3773-3781
(3) "Polymorphisms of neonatal Fc receptor in cynomolgus and rhesus macaques"
Uno, Utoh, Iwasaki
Drug Metab Pharmacokinet (2014) 29 (5), 427-30
Showing 1-3 of 4 papers.
Please refer to product data sheet.