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Recombinant Biotinylated Cynomolgus / Rhesus macaque FcRn/FCGRT & B2M, Avi Tag (Avitag™), 25 µg  

Recombinant Biotinylated Cynomolgus / Rhesus macaque FcRn/FCGRT & B2M, Avi Tag (Avitag™), 25 µg

MABSol® Recombinant Biotinylated Cynomolgus / Rhesus macaque FcRn / FCGRT & B2M Heterodimer Protein, expressed from human HEK293 cells, Avi tag (Avitag™), His-Tag, Strep II tag, (BLI verified)

Synonyms: recombinant, cynomolgus, protein, biotinylated, FcRn, FCGRT & B2M

More details

FCM-C82W5-25

420,00 €

Background: FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source:
Recombinant MABSol® Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified) (FCM-C82W5) is expressed from human HEK293 cells. It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the AA sequence of Cynomolgus and Rhesus macaque FcRn (FCGRT & B2M) are homologus.
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag&Strep II Tag (BLI verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.0 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 40 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Bioactivity
Please refer to product data sheet.

References

(1) Goebl NA., et al., 2008, Mol. Biol. Cell 19 (12): 5490–505.
(2) Lee TY., et al., 2008, Clin. Cancer Res. 14 (5): 1487–93.
(3) Güssow D., et al., 1987, J. Immunol. 139 (9): 3132–8.
(4) Gorevic P.D., et al., 1986, Proc. Natl. Acad. Sci. U.S.A. 83:7908-7912.

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