Recombinant Biotinylated Human PD-L2 / B7-DC Protein (recommended for biopanning), Avi Tag (Avitag™), His Tag, 25µg

Background
Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Source
Recombinant Biotinylated Human PD-L2, Avitag,His Tag (recommended for biopanning) (PD2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).
Predicted N-terminus: Leu 20

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 25.2 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Application
PD2-H82E8 works best for experiments that test the binding between PD-L2 and candidate antibodies, such as biopanning and other relevant assays.
This product is NOT suitable for testing PD1-PDL2 binding. For this type of application, we strongly recommend you to choose PD2-H82F6 as an alternative.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Balancing the Nanoscale Organization in Multivalent Materials for Functional Inhibition of the Programmed Death-1 Immune Checkpoint"
Paloja, Weiden, Hellmeier et al
ACS Nano (2023)
(2) "Comprehensive analysis of single-cell and bulk RNA-sequencing data identifies B cell marker genes signature that predicts prognosis and analysis of immune checkpoints expression in head and neck squamous cell carcinoma"
Wusiman, Li, Guo et al
Heliyon (2023) 9 (12), e22656
(3) "Linking tumor immune infiltrate and systemic immune mediators to treatment response and prognosis in advanced cervical cancer"
Rocha Martins, Luciano Pereira Morais, de Lima Galdino et al
Sci Rep (2023) 13 (1), 22634
Showing 1-3 of 1547 papers.

Citations

(1) "In Vitro Assays to Study PD-1 Biology in Human T Cells"
Authors: Tocheva, Anna S et al.
Journal: Current protocols in immunology vol 2020
Application: Flow cytometry
(2) "Anti-pd-1 antibodies and methods of use thereof"
Authors: Yasmina Noubia Abdiche, et al.
Journal: US20160159905A1 2016
Application: Flow Cytometry