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ActiveMax® Recombinant Human IL-6 protein, 50µg  

ActiveMax® Recombinant Human IL-6 protein, 50µg

ActiveMax® Recombinant Human IL-6 Protein, Val 30 - Met 212, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, IL6,HGF, Interleukin-6, BSF2, HSF, IFNB2

More details

IL6-H4218-50

Availability: within 7 days

420,00 €

Background
Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Source
ActiveMax® Human IL-6, Tag Free (IL6-H4218) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000591).
Predicted N-terminus: Val 30

Molecular Characterization
This protein carries no "tag". The protein has a calculated MW of 20.8 kDa. The protein migrates as 25-32 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>98% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
 
Bioactivity ELISA
Immobilized ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, Avitag,His Tag (Cat. No. CD6-H82E8) with a linear range of 4-125 ng/mL (QC tested).

Bioactivity-Cell based assay
ActiveMax® Human IL-6, Tag Free (Cat. No. IL6-H4218) stimulates proliferation of TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2856-0.3636 ng/mL (Routinely tested).

References

(1) Ferguson-Smith AC, et.al. 1988, Genomics, 2 (3): 203–208.
(2) Schwantner A,et. al. 2004, J. Biol. Chem. 279 (1): 571–6.
(3) Schuster B,et. al. 2003, J. Biol. Chem. 278 (11): 9528–35.
(4) Taga T,et al.1989, Cell 58 (3): 573–81.