Recombinant Biotinylated Human FGF basic Protein, Avitag™,His Tag (MALS verified), 25µg

Background
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.

Source
Recombinant Biotinylated Human FGF basic, Avitag,His Tag (FGC-H81E3) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
Predicted N-terminus: Met

Molecular Characterization
This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE).

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "The impact of prior SARS-CoV-2 infection on host inflammatory cytokine profiles in patients with TB or other respiratory diseases"
Cottam, Manneh, Gindeh et al
Front Immunol (2023) 14, 1292486
(2) "Associations of the circulating levels of cytokines with risk of ankylosing spondylitis: a Mendelian randomization study"
Ye, Wang, Zhong et al
Front Immunol (2023) 14, 1291206
(3) "Circulating inflammatory biomarkers and risk of intracranial aneurysm: a Mendelian randomization study"
Fang, Cao, Ni
Eur J Med Res (2024) 29 (1), 17
Showing 1-3 of 3789 papers.