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Recombinant Human FGF acidic Protein, Tag Free, 200 µg  

Recombinant Human FGF acidic Protein, Tag Free, 200 µg

Recombinant Human FGF acidic Protein, Phe 16 - Asp 155, produced in E.coli cells, Tag Free

Synonym: Recombinant human protein, FGF1, AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1, Heparin-binding growth factor

More details

AFF-H4116-200

Availability: within 7 days

234,00 €

Background
Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF acidic is a potent growth factor for fibroblasts and endothelial cells. FGF acidic is involved in wound repair, angiogenesis, and development. FGF acidic is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism. The ability of FGF acidic to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling. There are four distinct FGF receptors and each has multiple splice variants. FGF acidic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways.

Source
Recombinant Human FGF acidic, Tag Free (AFF-H4116) is expressed from E.coli cells. It contains AA Phe 16 - Asp 155 (Accession # P05230-1).
Predicted N-terminus: Met

Molecular Characterization
This protein carries no "tag"
The protein has a calculated MW of 16.0 kDa. The protein migrates as 16 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "A Novel Heterozygous Mutation c.1627G>T (p.Gly543Cys) in the SLC34A1 Gene in a Male Patient with Recurrent Nephrolithiasis and Early Onset Osteopenia: A Case Report"
Giusti, Marini, Al-Alwani et al
Int J Mol Sci (2023) 24 (24)
(2) "Recent developments in diagnosing bile acid diarrhea"
Lupianez-Merly, Dilmaghani, Camilleri
Expert Rev Gastroenterol Hepatol (2023)
(3) "PFOS-elicited metabolic perturbation in liver and fatty acid metabolites in testis of adult mice"
Lee, Lam, Tang et al
Front Endocrinol (Lausanne) (2023) 14, 1302965
Showing 1-3 of 2169 papers.