Recombinant human Aurora-B, protein kinase, 10 µg

Background: Aurora kinases play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. While only one kinase is present in yeast, three different aurora kinases have been found so far in mammalian cells (AuroraA, AuroraB and AuroraC). The three mitogenic serine/threonine kinases fulfil different functions. Whereas AuroraA plays important roles in centrosome maturation and bipolar spindle formation, AuroraB is required for chromosome bi-orientation and cytokinesis. The third member, AuroraC, is specifically expressed in male germline during meiosis. The three human kinases share a very conserved C-terminal catalytic domain, but each of them possesses an N-terminal that is different in size and in sequence. AuroraA and AuroraB are coincidently overexpressed in various human cancers, suggesting that aurora kinases may play a role in oncogenic transformation.

Human protein kinase Aurora B, human Aurora-B amino acids A2-A344, N-terminally fused to GST-HIS6

Theoretical MW: 70.0 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purity : >90%
Activation: This kinase was not activated by special procedures
Storage buffer: 50 mM HEPES pH 7.5,100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Storage temperature: - 80°C (avoid repeated freeze-thaw cycles!)
Shipping conditions: Dry ice
Protein concentration: 0.196 mg/ml (Bradford method using BSA as standard protein)
Specific activity: 37, 000 pmol/mg x min

Entrez Gene ID: 9212 
UniProtKB:  Q96GD4

Ordering information: shipped on dry ice

Product specific literature references:

Gautschi O, Heighway J, Mack PC, Purnell PR, Lara PN Jr, Gandara DR. (2008) "Aurora kinases as anticancer drug targets. Clin Cancer Res. 14(6):1639-48.

Barr AR, Gergely F. (2007) "Aurora-A: the maker and breaker of spindle poles." J Cell Sci. 120(Pt 17):2987-96.

Taylor S, Peters JM. (2008) "Polo and Aurora kinases: lessons derived from chemical biology." Curr Opin Cell Biol.; 20(1):77-84.