Recombinant human Aurora-C, protein kinase,10 µg

Aurora kinases play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. While only one kinase is present in yeast, three different aurora kinases have been found so far in mammalian cells (AuroraA, AuroraB and AuroraC). The three mitotic serine/threonine kinases fulfil different functions. Whereas AuroraA plays important roles in centrosome maturation and bipolar spindle formation, AuroraB is required for chromosome bi-orientation and cytokinesis. The third member, AuroraC, is specifically expressed in male germline during meiosis. The three human kinases share a very conserved C-terminal catalytic domain, but each of them possesses an N-terminal that is different in size and in sequence. AuroraA and AuroraB are coincidently overexpressed in various human cancers, suggesting that aurora kinases may play a role in oncogenic transformation.

Human protein kinase Aurora C, serine threonine kinase, amino acids M1-S275, recombinant and active enzyme, purity> 90 % by SDS-PAGE

Theoretical MW: 58.5 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol
Protein concentration: 0.116 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity: 4,000 pmol/mg x min

Entrez Gene ID: 6795 
UniProtKB:  Q9UQB9

Ordering information: shipped on dry ice

Product specific literature references:

Gautschi O, Heighway J, Mack PC, Purnell PR, Lara PN Jr, Gandara DR. (2008) "Aurora kinases as anticancer drug targets. Clin Cancer Res. 14(6):1639-48.

Barr AR, Gergely F. (2007) "Aurora-A: the maker and breaker of spindle poles." J Cell Sci. 120(Pt 17):2987-96.

Taylor S, Peters JM. (2008) "Polo and Aurora kinases: lessons derived from chemical biology." Curr Opin Cell Biol.; 20(1):77-84.