Recombinant Vascular endothelial growth factor receptor 1 (VEGF-R1), protein kinase domain, 10 µg

Background: Vascular Endothelial Growth Factor Receptor 1 (VEGFR1, Flt1), VEGFR2 (KDR/Flk1) and VEGFR3 (Flt4) are receptor tyrosine kinases (RTK) which are involved in the regulation of blood and lymphatic vessel development and homeostasis. All three kinases contain a large extracellular region composed of seven Ig-like domains (D1–D7), a single transmembrane (TM) helix and cytoplasmic region with a tyrosine kinase activity, and additional regulatory sequences. The second and third Ig-like domains, D2 and D3, function as binding sites for the VEGF family of cytokines (i.e., VEGF-A, -B, -C, -D, and placenta growth factor). Whereas VEGFR2 is expressed mainly in endothelial cells, VEGFR1 is expressed also in hematopoietic stem cells and inflammatory cells, such as monocytes and macrophages, in which it regulates chemotaxis. Signal transduction induced by VEGFR-2 and VEGFR-3 drive angiogenesis, whereas VEGFR-1 mostly acts to restrict the angiogenic response and recruit macrophages for tissue remodeling.

Purified recombinant human VEGF-R1, amino acids K784-I1338, expressed in Baculovirus infected Sf9 cells, N-terminally fused to GSTFactor Xa cleavage site

Theoretical MW : 89.4 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.25 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 53,000 pmol/mg x min 

Entrez Gene ID: 2321
UniProtKB:   P17948

Ordering information: shipped on dry ice

Product specific literature: 

Amano H, Kato S, Ito Y, Eshima K, Ogawa F, Takahashi R, Sekiguchi K, Tamaki H, Sakagami H, Shibuya M, Majima M. (2015)  “The Role of Vascular Endothelial Growth Factor Receptor-1 Signaling in the Recovery from Ischemia.” PLoS One. 2015;10(7):e0131445.

Yang Y, Xie P, Opatowsky Y, Schlessinger J.(2010) “Direct contacts between extracellular membrane-proximal domains are required for VEGF receptor activation and cell signaling.” Proc Natl Acad Sci U S A. 107(5):1906-11.

Jeltsch M, Leppänen VM, Saharinen P, Alitalo K.(2013)  “Receptor tyrosine kinase-mediated angiogenesis.”Cold Spring Harb Perspect Biol. 1;5(9)