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Recombinant Human Siglec-3 / CD33 Protein, Llama IgG2b Fc Tag, low endotoxin, 100 µg  

Recombinant Human Siglec-3 / CD33 Protein, Llama IgG2b Fc Tag, low endotoxin, 100 µg

Recombinant human Siglec-3, Asp 18 - His 259, expressed from human 293 cells (HEK293), Llama IgG2b Fc Tag

Synonym: recombinant, human, protein, CD33, SIGLEC3, gp67

More details


420,00 €

Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

Recombinant Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin (CD3-H5259) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18

Molecular Characterization
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 54.6 kDa. The protein migrates as 64-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 0.01 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) at 2 μg/mL (100 μL/well) can bind Human DcR3, Fc Tag (Cat. No. TNB-H5255) with a linear range of 0.01-0.3 μg/mL (QC tested).


(1) Garnache-Ottou F., et al., 2005, Blood 105 (3): 1256–64.
(2) Hernández-Caselles T, et al., 2006, J. Leukoc. Biol. 79 (1): 46–58.
(3) Walter RB, et al., 2007, Blood 109 (10): 4168–70.
(4) Ulyanova, T. et al., 1999, Eur. J. Immunol. 29:3440.
(5) Crocker, P.R. and A. Varki, 2001, Immunology 103:137.