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Recombinant Biotinylated Human FcRn / FCGRT & B2M, ultra sensitivity (primary amine labeling), 200 µg  

Recombinant Biotinylated Human FcRn / FCGRT & B2M, ultra sensitivity (primary amine labeling), 200 µg

MABSol® Recombinant Biotinylated Human FcRn / FCGRT & B2M, expressed from human HEK293 cells, His Tag (FcRN), Strep II tag (B2M)

Synonyms: recombinant human, protein FcRN & B2M,FCGRT & B2M

More details

FCM-H8286-200

1 380,00 €

Background: FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
MABSol® Biotinylated Human FcRn / FCGRT & B2M (FCM-H8286) is expressed from human HEK293 cells. It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_001129491.1 (FCGRT) & AAH32589 (B2M)). It is the biotinylated form of Human FcRn / FCGRT & B2M Heterodimer Protein (Cat # FCM-H5286).
Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)

Molecular Characterization
FcRN(Ala 24-Ser 297 ), NP_001129491.1, polyhistidine B2M(Ile 21-Met 119) AAH32589 Strep II
The subunit FcRN carries a polyhistidine tag at the C-terminus.
The subunit B2M carries a Strep II tag at the C-terminus.
The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein
Biotinylated Human ErbB3 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. LIT-H5256) at 2 μg/mL (100 μL/well) can bind Human HVEM, Fc Tag (Cat. No. HVM-H5258) with a linear range of 0.3-20 ng/mL (QC tested). Immobilized Human HVEM, Fc Tag (Cat. No. HVM-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA, Fc Tag (Cat. No. BTA-H82F8) with a linear range of 0.156-5 μg/mL (Routinely tested).

References

(1) Goebl NA., et al., 2008, Mol. Biol. Cell 19 (12): 5490–505.
(2) Lee TY., et al., 2008, Clin. Cancer Res. 14 (5): 1487–93.
(3) Güssow D., et al., 1987, J. Immunol. 139 (9): 3132–8.
(4) Gorevic P.D., et al., 1986, Proc. Natl. Acad. Sci. U.S.A. 83:7908-7912.

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