Recombinant Human Glypican 3 / GPC3 Protein, Fc Tag, low endotoxin, 100 µg

Background
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Source
Recombinant Human Glypican 3, Fc Tag, low endotoxin (GP3-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).
Predicted N-terminus: Gln 25 & Ser 359

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 49.3 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "CpG methylation changes in human mesenchymal and neural stem cells in response to in vitro niche modifications"
Gyimesi, Oikari, Yu et al
Biochimie (2024)
(2)  "Synthesis and preclinical evaluation of a novel probe [18F]AlF-NOTA-IPB-GPC3P for PET imaging of GPC3 positive tumor"
Mo, Sun, Liang et al
Bioorg Chem (2024) 147, 107352
(3) "A dual-signal output electrochemical aptasensor for glypican-3 ultrasensitive detection based on reduced graphene oxide-cuprous oxide nanozyme catalytic amplification strategy"
Li, Feng, Li et al
Bioelectrochemistry (2024) 158, 108709
Showing 1-3 of 2016 papers.