Recombinant human Misshapen-like kinase 1 (MINK1), protein kinase domain, 10 µg

Background: Misshapen/NIKs-related kinase (MINK1) belongs to the Ste20 group of kinases and is further classified as a member of the germinal center kinase (GCK) family. Several studies demonstrated that MINK1 is essential for cytoskeletal organization and oncogene-induced cell senescence. Furthermore, MINK1 is a potential mitotic kinase that regulates abscission. MINK1 can form a complex with PP2A holoenzyme and is directly associated with STRN4, a regulatory subunit of PP2A. Thus results in a decreased catalytic activity of MINK1 may be required for the dephosphorylation of abscission factors to promote abscission.

Recombinant human MINK1 (misshapen-like kinase 1), amino acids M1-E320, protein kinase domain, recombinant and active enzyme, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells

Theoretical MW : 67,100 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced
glutathione, 20% glycerol
Protein concentration: 0.297 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 95,000 pmol/mg min

Entrez Gene ID: 50488
UniProtKB: Q8N4C8

Ordering information: shipped on dry ice

Product specific literature

Hyodo T, Ito S, Hasegawa H, Asano E, Maeda M, Urano T, Takahashi M, Hamaguchi M, Senga T. (2012) “Misshapen-like kinase 1 (MINK1) is a novel component of striatin-interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis.” J Biol Chem. 287(30):25019-29

Nicke B., Bastien J., Khanna S. J., Warne P. H., Cowling V., Cook S. J., Peters G., Delpuech O., Schulze A., Berns K., Mullenders J., Beijersbergen R. L., Bernards R., Ganesan T. S., Downward J., Hancock D. C. (2005) Involvement of MINK, a Ste20 family kinase, in Ras oncogene-induced growth arrest in human ovarian surface epithelial cells. Mol. Cell. 20, 673–685

Dan I., Watanabe N.M., Kobayashi T., Yamashita-Suzuki K., Fukagaya Y., Kajikawa E., Kimura W.K., Nakashima T.M., Matsumoto K., Ninomiya-Tsuji J., Kusumi A. (2000) "Molecular cloning of MINK, a novel member of mammalian GCK family kinases, which is up-regulated during postnatal mouse cerebral development." FEBS Lett. 469:19-23