Recombinant human Mammalian STE20-like protein kinase 4 (MST4), 10 µg

Background: The mammalian Ste20 family consists of two structurally distinct subfamilies, including the P21-activated kinase family (PAK) and the germinal center kinase (GCK) family. MST1 and MST2 are members of the GCK II subfamily. MST4 belong as well as MST3 to the GCK III subfamily and is highly expressed in placenta, thymus, and peripheral blood leukocytes. MST4 is composed of an N-terminal kinase domain and a C-terminal regulatory domain which mediates homodimerization, and influences kinase activation. MST4 may activate the extracellular signal-regulated kinase (ERK) signaling pathway and acts downstream of liver kinase B1 (LKB1), a  heterotrimeric protein consisting of the scaffold protein MO25 and the catalytically inactive pseudokinase STRAD (STE20-related adaptor). Mst4 translocates from the Golgi to the subapical membrane compartment upon activation of Lkb1, phosphorylates the regulatory T567 residue of Ezrin and induce brush border formation.

Human MST4 (mammalian sterile 20-like 4), recombinant and active enzyme, full lenght, amino  acids M1-P416, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW : 76.424 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.209 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity : 18,000 pmol/mg min

Entrez Gene ID: 51765 
UniProtKB: Q9P289

Ordering information: shipped on dry ice

Product specific literature:

Filippi BM1, de los Heros P, Mehellou Y, Navratilova I, Gourlay R, Deak M, Plater L, Toth R, Zeqiraj E, Alessi DR.(2011) “MO25 is a master regulator of SPAK/OSR1 and MST3/MST4/YSK1 protein kinases.” EMBO J.30(9):1730-41.

Shi Z1, Jiao S, Zhang Z, Ma M, Zhang Z, Chen C, Wang K, Wang H, Wang W, Zhang L, Zhao Y, Zhou Z.(2013) „Structure of the MST4 in complex with MO25 provides insights into its activation mechanism.” Structure. 2013 Mar 5;21(3):449-61.

Sugden PH1, McGuffin LJ, Clerk A.(2013) “SOcK, MiSTs, MASK and STicKs: the GCKIII (germinal centre kinase III) kinases and their heterologous protein-protein interactions.” Biochem J. 2013 Aug 15;454(1):13-30.

Ling P1, Lu TJ, Yuan CJ, Lai MD.(2008) “Biosignaling of mammalian Ste20-related kinases.” Cell Signal. 2008 Jul;20(7):1237-47