Recombinant human Mammalian Sterile20-like kinase 1 (Mst1), 10 µg

Background: Mammalian Sterile20-like kinase 1 (Mst1) is a serine/threonine kinase which represents a member of the germinal center kinases (GCKs), a family of ‘Sterile 20 (STE20) like kinases’, which participate in both the determination of cell fate and the regulation of cell functions. MST1 and MST2 are mammalian homologs of drosophila ‘Hpo’. The MST1 /STEK4 contains an N-terminal catalytic domain, followed by an autoinhibitory segment and a coiled-coil SARAH domain that mediates hetero- and homo-dimerisation. MST1 has been implicated in T-cell biology. Thus, cross-talk between MST1 and Akt appears able to antagonize Akt1 activity which is involved in T-cell co-stimulation and the regulation of NF-κB-dependent gene transcription. MST1/2 in conjunction with an adaptor protein Salvador phosphorylates and activates LATS1/2 kinases which finally results in an inhibition of cell proliferation and promotion of apoptosis.

Recombinant human MST1, amino acids M3-F487, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells

Theoretical MW : 86.340 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced
glutathione, 20% glycerol
Protein concentration: 0.306 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 322,000 pmol/mg min

Entrez Gene ID: 6789
UniProtKB: Q13043

Ordering information: shipped on dry ice

Product specific literature:

Avruch J1, Zhou D, Fitamant J, Bardeesy N. (2011) ”Mst1/2 signalling to Yap: gatekeeper for liver size and tumour development.” Br J Cancer. ;104(1):24-32.

Yin H1, Shi Z, Jiao S, Chen C, Wang W, Greene MI, Zhou Z. (2012) “Germinal center kinases in immune regulation.” Cell Mol Immunol. (6):439-45.

de Souza PM1, Lindsay MA. (2004) „Mammalian Sterile20-like kinase 1 and the regulation of apoptosis.” Biochem Soc Trans. (Pt3):485-8.

Liu G1, Shi Z2, Jiao S2, Zhang Z1, Wang W2, Chen C2, Hao Q, Zhang M2, Feng M2, Xu L2, Zhang Z2, Zhou Z3, Zhang M4. (2014) Structure of MST2 SARAH domain provides insights into its interaction with RAPL.J Struct Biol.185(3):366-74.