Recombinant human Mitogen-activated protein kinase kinase kinase 1 (MAP3K1), 10 µg

Background: Mitogen-activated protein kinases (MAPKs) are a family of protein kinases that regulate a multitude of cellular responses including cell death, proliferation, and differentiation. MAPKs are regulated by the upstream phosphorylation of MAP2Ks, which in turn are regulated by phosphorylation through the MAP3Ks. MAP3K1, a 195 kDa protein with a C-terminal protein kinase domain (serine and threonine specific) acts as a MAP kinase kinase kinase and phosphorylates several different MEKs, with the strongest activity toward MKK4, the upstream kinase for JNKs. Furthermore, it contains a large noncatalytic N-terminus with a plant homeodomain (PHD) that shares significant homology to a Really Interesting New Gene (RING) motif, and by these means can regulate both the MAPK signaling pathways and the ubiquitin (Ub) proteasome system. As such, Map3k1 has complex roles in the regulation of cell death, survival, migration and differentiation.

Purified recombinant human  Human MAP3K1, C-terminal fragment, amino acids M1193-W1512 (as in NCBI/Protein entry NP_005912.1), expressed in Baculovirus infected Sf9 cells, N-terminal 4xFLAG,C-terminal HIS8-fusion protein

Theoretical MW: 41.39 kDa
Expression system: Baculovirus infected Sf9 cells
Purification: Affinity purification using Immobilized Metal Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.48 mg/ml (Bradford method using BSA as standard protein)
Activation: in vitro autoactivation
Method for determination of Km value & specific activity: Filter binding assay MSPH membrane
Specific activity: 5,300 pmol/mg x min (Substrate: Bio-RS-Peptide)

Entrez Gene ID: 4214
UniProtKB: Q13233

Ordering informations: shipped on dry ice

Product specific literature references

Lu Z., Xu S., Joazeiro C., Cobb MH., Hunter T. (2002) “The PHD domain of MAP3K1 acts as an E3 ubiquitin ligase and mediates ubiquitination and degradation of ERK1/2”. Mol Cell  9:945-56

Suddason T, Gallagher E.(2015) “A RING to rule them all? Insights into the Map3k1 PHD motif provide a new mechanistic understanding into the diverse roles of Map3k1.” Cell Death Differ.22(4):540-8

Gallagher E, Suddason T.(2015) “The PHD motif of Map3k1 activates cytokine-dependent MAPK signaling.” Mol Cell Oncol. 2015 May 6;2(3)