8-Br-cAMP, 100 µmol

Background: The second messenger cyclic AMP activates the holo enzyme complex of the cAMP dependent protein kinase PKA, the Rap1 guanine-nucleotide-exchange factor Epac, cAMP-gated ion channels, CAP protein and some other target receptors. The 8-Br-cAMP has an increased hydrolytical stability and membrane permeability compared to unmodified cAMP.

Chemical name.:        8-Bromoadenosine- 3',5'-cyclic monophosphate (8-Br-cAMP)
CAS number:            76939-46-3
Formula:                   C10H10BrN5O6P Na
Molecular Weight:     430.1 g/mol
Long Term Storage:    -20°C
Purity:                          >98%
Appearance:               White lyophilized powder
Solubility:                     water (200 mM), DMSO (200 mM) Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.

Product specific literature references:

Ogreid D, Ekanger R, Suva RH, Miller JP, Doskeland SO (1989) "Comparison of the two classes of binding sites (A and B) of type I and type II cyclic-AMP-dependent protein kinases by using cyclic nucleotide analogs" Eur. J. Biochem. 181(1):19-31

Ringler GE, Kao LC, Miller WL, Strauss JF (1989) "Effects of 8-bromo-cAMP on expression of endocrine functions by cultured human trophoblast cells. Regulation of specific mRNAs" Mol. Cell. Endocrinol. 61(1):13-21

Schwede F, Christensen A, Liauw S, Hippe T, Kopperud R, Jastorff B, Doskeland SO (2000) "8-Substituted cAMP analogues reveal marked differences in adaptability, hydrogen bonding, and charge accommodation between homologous binding sites (AI/AII and BI/BII) in cAMP kinase I and II" Biochemistry 39(30):8803-12.

Product Citations:

Wang Y, Adjaye J.(2011) "A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells."Stem Cell Rev. 7(2):331-41.

Saito T, Sugimoto N, Ohta K, Shimizu T, Ohtani K, Nakayama Y, Nakamura T, Hitomi Y, Nakamura H, Koizumi S, Yachie A (2012) "Phosphodiesterase inhibitors suppress Lactobacillus casei cell-wall-induced NF-κB and MAPK activations and cell proliferation through protein kinase A--or exchange protein activated by cAMP-dependent signal pathway." ScientificWorldJournal:748572.

Sugimoto N, Miwa S, Ohno-Shosaku T, Tsuchiya H, Hitomi Y, Nakamura H, Tomita K, Yachie A, Koizumi S.  (2011) "Activation of tumor suppressor protein PTEN and induction of apoptosis are involved in cAMP-mediated inhibition of cell number in B92 glial cells." Neurosci Lett. 497(1):55-9.

Wemhöner K, Kanyshkova T, Silbernagel N, Fernandez-Orth J, Bittner S, Kiper AK, Rinné S, Netter MF, Meuth SG, Budde T, Decher N (2015) "An N-terminal deletion variant of HCN1 in the epileptic WAG/Rij strain modulates HCN current densities." Front Mol Neurosci. 2015 Nov 3;8:63.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.