8-Cl-cAMP, 10 µmol (~4 mg)

The second messenger cyclic AMP activates the holo enzyme complex of the cAMP dependent protein kinase PKA, the Rap1 guanine-nucleotide-exchange factor Epac, cAMP-gated ion channels, CAP protein and some other target receptors. The 8-Cl-cAMP is a site selective cyclic AMP analogue with high selectivity for protein kinase A type I. It induces growth inhibition and differentiation in cultured cancer cell lines.

Chemical name:         8-Chloroadenosine- 3', 5'-cyclic monophosphate (8-Cl-cAMP)
Formula:                    C10H10ClN5O6P . Na
Molecular Weight:     363.7 . 23.0 g/mol
Purity:                        >99%
Appearance:             White lyophilized powder
Solubility:                   water (200 mM), DMSO (200 mM) Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.

Product specific literature references:

Ally S, Tortora G, Clair T, Grieco D, Merlo G, Katsaros D, Ogreid D, Doskeland SO, Jahnsen T, Cho-Chung YS (1988) "Selective modulation of protein kinase isozymes by the site-selective analog 8-chloroadenosine 3\',5\'-cyclic monophosphate provides a biological means for control of human colon cancer cell growth" Proc. Natl. Acad. Sci. U S A 85(17):6319-22

Tortora G, Ciardiello F, Ally S, Clair T, Salomon DS, Cho-Chung YS (1989) "Site-selective 8-chloroadenosine 3\',5\'-cyclic monophosphate inhibits transformation and transforming growth factor alpha production in Ki-ras-transformed rat fibroblasts" FEBS Lett. 242(2):363-7

Rohlff C, Clair T, Cho-Chung YS (1993) "8-Cl-cAMP induces truncation and down-regulation of the RI alpha subunit and up-regulation of the RII beta subunit of cAMP-dependent protein kinase leading to type II holoenzyme-dependent growth inhibition and differentiation of HL-60 leukemia cells" J. Biol. Chem. 268(8):5774-82

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.