cAMP, 500 µmol (~176 mg)

The second messenger cyclic AMP activates the holo enzyme complex of the cAMP dependent protein kinase PKA, the Rap1 guanine-nucleotide-exchange factor Epac, cAMP-gated ion channels, CAP protein and some other target receptors. It is metabolized rapidly by cyclic nucleotide-dependent phosphodiesterases.

Chemical name:         Adenosine 3', 5'-cyclic monophosphate (cAMP)
Formula:                    C10H11N5O6P . Na
Molecular Weight:     351.2 g/mol
Long Term Storage:   -20°C
Purity:                         >99% (HPLC)
Appearance:              White solid
Solubility:                    water (200 mM), DMSO (200 mM) Please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.

Product specific literature references:

Scott JD (1991) "Cyclic nucleotide-dependent protein kinases" Pharmacol Ther. 50(1):123-45

de Rooij J, Zwartkruis FJ, Verheijen MH, Cool RH, Nijman SM, Wittinghofer A, Bos JL (1998) "Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP." Nature. 396(6710):474-7

Rich TC, Fagan KA, Nakata H, Schaack J, Cooper DM, Karpen JW (2000) "Cyclic nucleotide-gated channels colocalize with adenylyl cyclase in regions of restricted cAMP diffusion" J. Gen. Physiol. 116(2):147-61

de Rooij J, Rehmann H, van Triest M, Cool RH, Wittinghofer A, Bos JL (2000) "Mechanism of regulation of the Epac family of cAMP-dependent RapGEFs."  J. Biol. Chem. 275(27):20829-36

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.