DB-cAMP, 100 µmol

DB-cAMP is a membrane-permeable derivative of cyclic AMP which activates the holo enzyme complex of the cAMP dependent protein kinase PKA, the Rap1 guanine-nucleotide-exchange factor Epac, cAMP-gated ion channels, CAP protein and some other target receptors. This analogue releases butyrate due to intracellular and extracellular esterase action. Butyrate was shown to have distinct biological effects.

DB-cAMP :                        order-no. PKE-DBCA-100
Chemical name.:                N6, 2'-O-Dibutyryladenosine-3', 5'-cyclic monophosphate, sodium salt
CAS No.                            16980-89-5
Formula:                            C18H23N5O8P . Na
Molecular Weight:             491,4 g/mol
Purity:                               >97% (HPLC/UV 273nm)
Appearance:                    white solid
Solubility:                          water (200 mM), DMSO (200 mM), please rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.

synonyms: Dibutyryl-cAMP, DBcAMP, Bu2-cAMP, bt2-cAMP, Bucladesine, Actosin

Product specific literature references:

Kaukel E, Mundhenk K, Hilz H (1972) "N 6 -monobutyryladenosine 3\':5\'-mono phosphate as the biologically active derivative of dibutyryladenosine 3\':5\'-monophosphate in HeLa S3 cells" Eur. J. Biochem. 27(1):197-200

Prasad KN, Kumar S (1975) "Role of cyclic AMP in differentiation of human neuroblastoma cells in culture" Cancer 36(4):1338-43

Yusta B, Ortiz-Caro J, Pascual A, Aranda A (1988) "Comparison of the effects of forskolin and dibutyryl cyclic AMP in neuroblastoma cells: evidence that some of the actions of dibutyryl cyclic AMP are mediated by butyrate" J. Neurochem. 51(6):1808-18

Lando M, Abemayor E, Verity MA, Sidell N (1990) "Modulation of intracellular cyclic adenosine monophosphate levels and the differentiation response of human neuroblastoma cells" Cancer Res. 50(3):722-7

Drab M, Haller H, Bychkov R, Erdmann B, Lindschau C, Haase H, Morano I, Luft FC, Wobus AM (1997) "From totipotent embryonic stem cells to spontaneously contracting smooth muscle cells: a retinoic acid and db-cAMP in vitro differentiation model" FASEB J. (11):905-15.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.