Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Feline FcRn / FCGRT & B2M, His,Avitag™ (MALS & SPR verified), 25 µg  

Recombinant Biotinylated Feline FcRn / FCGRT & B2M, His,Avitag™ (MALS & SPR verified), 25 µg

Biotinylated Feline FcRn / FCGRT & B2M Heterodimer Protein, Ala 24 - Ser 297 (FCGRT) & Val 21 - Met 118 (B2M), expressed from human HEK293 cells, His,Avitag™ (MALS & SPR verified)

Synonyms: recombinant, feline, biotinylated, protein, FcRN & B2M,FCGRT & B2M

More details

FCN-F82W3-25

420,00 €

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
Recombinant Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag (FCN-F82W3) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Val 21 - Met 118 (B2M) (Accession # W5VRL5-1(FCGRT) & Q5MGS7-1(B2M)).
Predicted N-terminus: Ala 24 (FCGRT) & Val 21 (B2M)

Molecular Characterization
Biotinylated Feline FCGRT&B2M Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.0 kDa (FCGRT) and 11.5 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag. The reducing (R) protein migrates as 36-40 kDa (FCGRT) and 18-20 kDa (B2M) respectively due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.

Reconstitution
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) "Polymorphisms of neonatal Fc receptor in cynomolgus and rhesus macaques"
Uno, Utoh, Iwasaki
Drug Metab Pharmacokinet (2014) 29 (5), 427-30
(2) "Humanized FcRn mouse models for evaluating pharmacokinetics of human IgG antibodies"
Proetzel, Roopenian
Methods (2014) 65 (1), 148-53
Showing 1-2 of 2 papers.
Please refer to product data sheet.

The following products could also be interesting for you: