Recombinant human CDK8/CycC, active protein kinase, 10 µg

The Cdks are serine/threonine protein kinases which are predominantly involved in the progression of the cell cycle. Subsequently, CDK-cyclin complexes (CDK7, CDK8 and CDK9) were also identified as regulators of transcription. CDK8/CycC is part of the RNA polymerase II holoenzyme complex and phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. On the other hand, CDK8/CycC phosphorylates cyclin H, a component of the CDK activating kinase (CAK), and represses activated transcription by modulation of TFIIH activity.

Human cyclin-dependent protein kinase CDK8, coexpressed in complex with with human CycC.

Theoretical MW MW GST-CDK8: 83.0 kDa
Theoretical MW His-CyC: 38.0 kDa
Expression system: Baculovirus infected Sf9 cells
Purification: Immobilized Metal Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.262 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 27,000 pmol/mg x min

Entrez Gene ID: 1024/892 
UniProtKB: P49336/ P24863

Ordering information: shipped on dry ice

Product specific literature references:

Akoulitchev S, Chuikov S, Reinberg D. (2000)"TFIIH is negatively regulated by cdk8-containing mediator complexes." Nature 7; 407(6800):102-6

Pinhero R, Liaw P, Bertens K, Yankulov K. (2004) "Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase." Eur J Biochem.;271 (5):1004-14.

Leclerc V, Leopold P. (1996) "The cyclin C/Cdk8 kinase." Prog Cell Cycle Res. 2:197-204.